Extended Data Fig. 8: KG2032 CAR NK cells recognized and killed AML cells in vitro and in vivo.
a. CD107a degranulation measured by flow cytometry in CAR NK cells or control (non-transduced) NK cells upon co-culture with the indicated cells (n = 5 technical replicates from a single experiment). Experiments were performed with three different AML samples and those with two samples are shown. The result of the experiments with another AML sample is shown in Fig.7d. b. Cytotoxicity assay performed via flow cytometry. AML cells were co-cultured with either KG2032 CAR NK cells or control NK cells at an effector:target ratio of 3:1 for 48 h, and the percentages of AML cells were measured by staining with anti-CD34 or anti–CLL1 mAb. A single experiment was performed. Each combination of target and effector cells was tested in technical triplicate and one representative result is shown. c. 51Cr release assay to measure specific lysis of primary AML cells by KG2032 CAR NK cells or control NK cells (n = 5 technical replicates from a single experiment). E/T, effector/target. Experiments were performed with three different AML samples and those with two samples are shown. The result of the experiments with another AML sample is shown in Fig.7e. d, e. KG2032 CAR NK cells established from CB cells carrying KG2032-reactive HLA-DRB1 alleles. Data are representative of two independent experiments using different donors. d. Representative flow cytometric analysis of the expression of CD56, CD3, and CAR on KG2032 CAR NK cells. e. 51Cr release assay to measure specific lysis of the indicated target cells by KG2032 CAR NK cells shown in d or control NK cells (n = 3 technical replicates from a single experiment, repeated with similar results using different donors).
