Extended Data Fig. 7: Anti-PILRα monoclonal antibody C21 potently inhibits tumor growth and is relatively safe in humanized mouse xenograft models.

a,b, Tumor tissues from humanized MDA-MB-231 xenograft models described in Fig. 6a were collected on day 20 for flow cytometry analysis. CD8⁺ (CD45⁺CD3⁺CD8⁺), CD8⁺ T_EM (CD45⁺CD3⁺CD8⁺CD62L^-CD45RO⁺), and CD8⁺ T_EFF (CD45⁺CD3⁺CD8⁺CD62L^-CD45RO^-) TILs gated by anti-CD45 antibody conjugated with APC (APC-CD45), anti-CD3 antibody conjugated with PerCP/Cy5.5 (PerCP/Cy5.5-CD3), anti-CD8 antibody conjugated with PE/Cy7 (PE/Cy7-CD8), anti-CD62L antibody conjugated with Alexa Fluor 700 (AF700-CD62L), and anti-CD45RO conjugated with FITC (FITC-CD45RO) were further analyzed by staining of anti-Granzyme B antibody conjugated with BV421 (BV421-Granzyme B) and anti-IFNγ antibody conjugated with PE (PE-IFNγ), or anti-CD25 antibody conjugated with BV421 (BV421-CD25). Representative flow cytometry plots depicting the percentage of IFNγ⁺ and granzyme B⁺ (a) and CD25⁺ (b) cells are shown (n = 5 independent mice). c, The percentage of CD25⁺, IFNγ⁺, and granzyme B⁺ cells within CD8⁺ (CD45⁺CD3⁺CD8⁺), CD8⁺ T_EM (CD45⁺CD3⁺CD8⁺CD62L^-CD45RO⁺), and CD8⁺ T_EFF (CD45⁺CD3⁺CD8⁺CD62L^-CD45RO^-) subsets as described in (a) and (b) (n = 5 independent mice). d,g, Body weight of mice from the treatment as described in Fig. 6a (d) and Fig. 6h (g) (n = 5 independent mice). e,h, Representative gross morphology of major organs including heart, liver, spleen, lung, and kidney collected from mice in Fig. 6b (e) and Fig. 6i (h) (n = 5 independent mice). f,i, Representative H&E staining images of tissue sections from organs in panel e (f) and h (i) (n = 5 independent mice). Data are presented as mean ± s.e.m. c, P values were calculated using a one-way ANOVA with Tukey’s multiple comparisons test. f,i, Scale bar, 100 μm.

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