xtended Data Fig. 3: The dual tumour spatial distribution function is unique to palmitic acid surface-displayed nanoparticles. | Nature Cancer

xtended Data Fig. 3: The dual tumour spatial distribution function is unique to palmitic acid surface-displayed nanoparticles.

From: Concurrent inhibition of tumor growth and metastasis by a lipidated nanophotosensitizer tracing and disabling tumor extracellular vesicles

xtended Data Fig. 3

a,b, CLSM images of MDA-MB-231 cells after incubation with FP-RhB for 1 h. Scale bars, 50 μm. c, Schematic of the construction of nanoparticles with different fatty acids (acetic acid, SA; hexanoic acid, HA; decanoic acid, DA; palmitic acid, PA) displaying on their surface. d,e, The particle sizes (d) and zeta potentials (e) of NP1, NP4, NP5, and NP6. f, CLSM examination of the cellular uptake and exocytosis behaviors of NP1, NP4, NP5, and NP6 in MDA-MB-231 cells after 60 min of incubation. Scale bar, 50 μm. g, Quantification of the fluorescence intensity of MDA-MB-231 cells with different treatments. h, Quantitative analysis of the fluorescence intensity of TEVs obtained from MDA-MB-231 after treatment with NP1, NP4, NP5, or NP6 for 1 h, or without treatment (termed TEVs), respectively. The protein concentrations of TEVsNP1, TEVsNP4, TEVsNP5, TEVsNP6, and TEVs were 2 mg/mL. i, Schematic of the preparation of the traditional tumour-targeting nanoparticles, for example Ctrl-NP1@RGD. j,k, The particle size (j) and zeta potentials (k) of Ctrl-NP1 and Ctrl-NP1@RGD. l, CLSM examination of the cellular uptake of Ctrl-NP1 and Ctrl-NP1@RGD in MDA-MB-231 cells after 60 min of incubation. Scale bars, 50 μm. m, Quantification of the fluorescence intensity of MDA-MB-231 cells after treatment with Ctrl-NP1 or Ctrl-NP1@RGD for 1 h. n, 3D confocal Z-stack images of MDA-MB-231 cells treated with Ctrl-NP1@RGD for 1 h. Scale bar, 50 μm. In all experiments, the concentration of NPs was 0.1 g/L. In d,e,h,j,k, n = 3 biologically independent samples; in g,m, n = 4 biologically independent samples. Data are presented as the mean ± s.d. Statistical significance was calculated via one-way ANOVA with Tukey’s multiple comparisons test (g,h) and unpaired two-tailed Student’s t-test (m). In a-b,d-h,j-n, experiments were repeated three times independently with similar results; representative images are shown in a,b,f,l,n.

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