Extended Data Fig. 6: Impact of TAMs on human HR+ breast cancer.
a, Mean distance of PD-L1+/PD-L1− macrophages and tumor cells to the nearest γδ T cell in diagnostic biopsies of HR+HER2− BC (n = 26) assessed by multispectral imaging. Box-plots overlaid by individual data points show medians ± interquartile range; whiskers indicate minima and maxima. Relevant and significant (P < 0.05) statistical differences (by two-tailed paired t-test) are shown. Scale bar, 100 μm b, UMAPs for macrophage clusters by scRNAseq, color-coded for the indicated cell type, from longitudinal HR+HER2− BC diagnostic biopsies of responder and non-responder patients receiving preoperative pembrolizumab (α-PD-1) followed by (→) RT plus α-PD-1. Data are pooled from 36 biopsies obtained from 12 patients (3 each: at baseline, after PD-1 and at surgery).
