Extended Data Fig. 5: Heterogeneity of fibroblast subpopulations in cancerous tissues.
Related to Fig. 3. (a) Heatmap showing the ORs of cell types occurring in each source. OR > 1.5 indicates that the cell type is preferred to distribute in the corresponding source. Significance was assessed using the two-sided Fisher test and adjusted using the BH method. (b) Left: heatmap showing Jaccard similarity indices for comparisons among 1,470 robust NMF programs based on their top 50 genes within the fibroblast cell lineage. Programs are ordered by clustering and grouped into families of MPs with related functions (marked by black dashed lines); MP families are numbered and labeled. Right: list of all MP names, separated into MP families. (c) Box plot depicting the signature scores of MPs across fibroblast subtypes, calculated from 379 samples. MPs are color-coded based on their functional annotations. The bottom of the box represents the Q1, and the top of the box represents the Q3. The height of the box represents the IQR, while the horizontal line inside the box indicates the median. The whiskers extend to the positions of Q1 - 1.5 * IQR and Q3 + 1.5 * IQR. (d) Heatmap illustrating the GSVA scores assigned to metabolic pathways within each fibroblast subset, with the pathways categorized according to KEGG nomenclature.
