Fig. 3: CTHRC1+ fibroblasts are broadly present in tumor datasets and highly express ECM-remodeling-associated genes. | Nature Cancer

Fig. 3: CTHRC1+ fibroblasts are broadly present in tumor datasets and highly express ECM-remodeling-associated genes.

From: Spatiotemporal analyses of the pan-cancer single-cell landscape reveal widespread profibrotic ecotypes associated with tumor immunity

Fig. 3

a, UMAP visualization of fibroblast MetaCell distribution, colored by cell type. b, Dot plot depicting the expression of representative signature genes of each fibroblast cell type. c, Scatter plot showing ECM remodeling, immunoregulatory and antigen-presenting signature scores for each fibroblast subset; Imm-Reg, immune-regulatory. d, Box plot showing the proportion of each fibroblast cell type in different source-derived samples (normal, precancerous, tumor and metastatic tissue) from 338 treatment-naive samples. Significance labels in the figure were assessed via Kruskal–Wallis tests to compare each cell type distribution among four tissue types. Significance for pairwise source proportions within each cell type, assessed via two-tailed unpaired Wilcoxon tests, is reported in Supplementary Table 6. The open rectangle annotates the comparative scope, with BH correction for multiple testing. The bottom of the box represents Q1, and the top of the box represents Q3. The height of the box represents the IQR, whereas the horizontal line inside the box indicates the median. The whiskers extend to positions of Q1 – 1.5 × IQR and Q3 + 1.5 × IQR. e, Heat map showing the proportion of different fibroblast cell types in various cancer types or healthy tissues. For rows, a bar plot illustrates the number of miniclusters (in log10 scale) and the origin of cancer cells labeled by the different colors. f, Heat map displaying the enriched pathways for each fibroblast subset. Enrichment was calculated using hypergeometric distribution statistics, with P values adjusted by the BH method; FDR, false discovery rate. g, Violin plot showing the glycosaminoglycan biosynthesis pathway and the average metabolic pathway GSVA score for each fibroblast subset across 379 samples. h, Kaplan–Meier plots demonstrate the clinical impact of eFibro_CTHRC1 cells in 533 individuals with KIRC and 405 individuals with BLCA, comparing low and high signature scores; +, censored observations. Statistical significance was assessed via the log-rank test, with P values of 0.00523 for KIRC and 0.00568 for BLCA.

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