Fig. 1: Results of in vitro experiments. | Communications Materials

Fig. 1: Results of in vitro experiments.

From: Bio-responsive polymers for dual 31P/19F-magnetic resonance to detect reactive oxygen species in vivo

Fig. 1

a Fluorescence measurement of ROS production in 4T1 cells using the nonfluorescent dye OxyBURST Green H2HFF BSA (OB), which is oxidized by ROS to the fluorescently active derivative. Results are presented as the total emission (photons∙s−1) in controls, normalized to the signal of 4T1 cells in a medium with 10 mg∙mL−1 OB over time. b 31P/19F -polymer in phantom (cp = 100 mM; V = 100 µL) measured using 19F- and 31P-MRS and 1H/19F/31P-MRI. 19F- (red) and 31P-(green) MRI data are overlaid with 1H-MRI signal. c Relative 31P-MRS amplitude decreases (%) in different ROS-triggered oxidative conditions: 31P-polymer with (left) H2O2 and (right) 4T1 cells, reflecting the oxidation of the P = S (∆δ ~ 60 ppm) signal to the P = O (∆δ ~ 0 ppm) signal over time.

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