Fig. 2: Results of in vivo experiments. | Communications Materials

Fig. 2: Results of in vivo experiments.

From: Bio-responsive polymers for dual 31P/19F-magnetic resonance to detect reactive oxygen species in vivo

Fig. 2

a Experimental schematic outlining the steps including tumor induction, injection of 31P/19F-polymer, and MR measurement setup. b MR chemical shift between the 31P/19F-polymer and anesthesia (isoflurane; ∆δ ~ 20 ppm, 19F-MRS), and between physiological signal (metabolites; ∆δ ~ 60 ppm, 31P-MRS). c Results of proof-of-principle in vivo 19F-MRI after retro-orbital 31P/19F-polymer injection. Overlaid 1H/19F-MRI with the fluorine signal highlighted in red. Results are visible 6 h (Day 0 + 6 h), 24 h (Day 1), and 48 h (Day 2) post-injection. d Results of in vivo 31P-MRS and 19F-MRS/MRI in response to ROS-triggered changes after I.T. 31P/19F-polymer injection. Overlaid 1H/19F-MRI and 19F-MRS with fluorine signal presented in red, and 31P-MRS data with the phosphorus signal highlighted in green; both measured 6- and 24-h post-injection (Day 0 + 6 h and Day 1). On the right, average SNR values of the 31P/19F-polymer injected I.T. for both 19F- and 31P-MRS; * indicates 31P-MRS SNR values below the detection limit (<1); both measured 0.5–48 h post-injection (Day 0 to Day 2).

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