Fig. 5: Anti-biofilm activity of HD5-myr and PVA@HD5-myr against drug-resistance bacteria in vitro.
From: Defensin-conjugated polymer fabrics combat drug-resistant biofilms and toxins in skin wounds
a–c Biofilm formation by (a) imipenem/cilastatin sodium-resistant A. baumannii, (b) carbapenems-resistant P. aeruginosa and (c) MRSA after 48 h incubation in bacteria medium containing 3.125–50 μg mL-1 of HD5-myr and HD5. Results are expressed as the percentage of biofilm growth for each sample relative to the positive control. Data are shown as mean ± SD and statistical significance was calculated by unpaired two-tailed Student’s t-test (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant. d–f SEM images of bacterial biofilms formation on PVA and PVA@HD5-myr dressings through incubation with (d) imipenem/cilastatin sodium-resistant A. baumannii, (e) carbapenems-resistant P. aeruginosa and (f) MRSA for 48 h (n = 3). Scales bars in left (350 ×), middle (2500 ×) and right (5000 ×) pictures are 50, 10, and 5 μm in length, respectively.
