Fig. 6: The protective effect of PVA@HD5-myr on skin wound in murine full-thickness wound models complicated with MRSA infection and Biofilm formation in vivo.

a-h Mice skin wound model complicated with MRSA infection. Before bacterial infection, the skin wounds of mice in the PVA group and the PVA@HD5-myr group were respectively covered with two pieces of PVA or PVA@HD5-myr nonwoven fabrics (2 × 2 cm). a Macroscopic imaging of wounds immediately following surgery (day 0) and after 5, 10, or 15 days of treatment in PVA treatment and PVA@HD5-myr treatment group. b Representative images of H&E staining and Masson’s trichrome staining of skin tissue sections from murine wound models. c Total murine skin histopathological scores according to results of H&E staining and Masson’s trichrome staining (n = 8). d Quantification of the positive area of collagen deposition according to Masson’s trichrome staining images (n = 8). e Bacterial burden analysis of murine regenerated skin tissues in PVA and PVA@HD5-myr group after modeling for 15 days (n = 8). f Analysis of body weight change in mice after modeling for 15 days (n = 8). g Formation of bacterial biofilms on PVA or PVA@HD5-myr dressings after modeling for 3 days (n = 5). Scales bars in left (1200 ×) and right (3000 ×) pictures are 10 and 4 μm in length, respectively. h Formation of bacterial biofilms on the skin surface of mice in PVA or PVA@HD5-myr group after modeling for 3 days (n = 5). Scales bars in left (2500 ×) and right (10000 ×) pictures are 5 and 1 μm in length, respectively. Data are shown as mean ± SD and statistical significance was calculated by unpaired two-tailed Student’s t-test for comparison between two experimental groups and one-way ANOVA analysis of variance with Bonferroni corrections for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant.