Fig. 7: The binding ability of HD5-myr to LPS in vitro and protective effect of PVA@HD5-myr on skin wound in murine full-thickness wound models complicated with endotoxin or P. aeruginosa in vivo.
From: Defensin-conjugated polymer fabrics combat drug-resistant biofilms and toxins in skin wounds
a Kaplan−Meier survival analysis of mice. A full-thickness back skin wound (1 × 1 cm) of BALB/c mice (n = 10) was constructed. Two pieces of PVA or PVA@HD5-myr nonwoven fabrics (2 × 2 cm) covered the wound bed and were fixed with thin transparent dressings. Then P. aeruginosa (ATCC 27853, 1 × 108 CFU in 50 µL PBS) was added. The survival of mice was continuously monitored for 72 h. The data were analyzed by the Mantel-Cox test. b,c Measurement of the binding affinities between HD5 or HD5-myr and LPS by SPR assay using Biacore. (b) HD5 or (c) HD5-myr were covalently immobilized on the CM5 sensor chip through their amine groups and LPS flowed over HD5 or HD5-myr. LPS was diluted to different concentrations (from 0.63 to 10 μM). The interactive parameters, including association constant (Ka), dissociation constant (Kd) and equilibrium dissociation constant (KD) were calculated by BIA evaluation software. The resulting data were fit to a 1:1 binding model. d–g Mice skin wound model complicated with endotoxin infection. Analysis of (d) endotoxin and cytokines including (e) TNF-α, (f) IL-1β and (g) IL-6 in skin wound of mice after modeling for 6 h (n = 13). Data are shown as mean ± SD and statistical significance was calculated by one-way ANOVA analysis of variance with Bonferroni corrections for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not significant.
