Fig. 4: Differentially transcribed genes of M. luminyensis CZDD1 in H2-dependent versus DIET growth.

Heat plot representation of the differential expression (Log2) in the mid-exponential methylotrophic cultures of M. luminyensis grown respectively under 0.1 MPa H2 at day 14 (M.l + H2), and in coculture with C. malenominatum at day 2 (C.m + M.l) (A). Blue and red represent the minima and maxima fold, respectively. Quantitative RT-PCR of some differentially transcribed genes involved in methanogenesis in the M. luminyensis monoculture (red bar), coculture (blue bar) and electrochemical culture (orange bar) (B). Three replicate experiments were performed, and the averages and standard deviations are shown in (B). Statistics are estimated by T-test, and ***, ** and ns indicate very significant (P < 0.01), significant (P < 0.05) and not significant, respectively.