Extended Data Fig. 7: Related Fig. 5; CXCL12 is predominantly secreted by p16h-sn iCAFs. | Nature Aging

Extended Data Fig. 7: Related Fig. 5; CXCL12 is predominantly secreted by p16h-sn iCAFs.

From: Preexisting senescent fibroblasts in the aged bladder create a tumor-permissive niche through CXCL12 secretion

Extended Data Fig. 7

a, UMAP showing the expression levels of Cxcl12 in Tomato+ (p16h-sn) cells from scRNA-seq data using the aged male mouse bladder. b, Violin plots showing the expression levels of Cxcl12 in Tomato+ (p16h-sn) cells of each cell type. c, qPCR of p16 and p21 using the sorted iCAFs from p16-CreERT2-Tomato mice. (n = 7 for each group). d, Scheme of MB49 culture using conditioned medium collected from Tomato−or whole iCAFs. e, The bright-field image of Tomato− (p16l) and Tomato+ (p16h-sn) iCAFs. The red color represented the fluorescent signals of Tomato. Scale bar, 100 μm. Paired t-test was performed for c. Data in c are presented as mean values ± SEM. All the t-test were two-side.

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