Fig. 6: Transcriptomic signature of p16h-sn CAFs as a prognostic factor in bladder cancer.
a, Heat map showing the expression levels of LUM, CRISPLD2, CXCL12, SMOC2, GUCY1A3 and GAS1 in TCGA bladder cancer data with clinical information (n = 436). b, Quantitative score of the p16h-sn CAF signature across different age groups of patients from TCGA dataset (age ≤64 years, n = 138; age 65–74 years, n = 133; age ≥75 years, n = 155). c, Overall survival probability of patients from TCGA. The patients were evenly divided into two groups based on the p16h-sn CAF signature score (low, n = 212; high, n = 213). d, Scores of the p16h-sn CAF signature in relation to the indicated clinical predictive factors among patients from TCGA dataset (metastasis, n = 139 (negative), n = 140 (positive); recurrence, n = 264 (negative), n = 129 (positive); pathological T stage, n = 127 (≤pT2), n = 204 (pT3), n = 61 (pT4); grade, n = 21 (low), n = 402 (high)). e, Hazard ratios and 95% confidence intervals of the p16h-sn CAF signature and the four clinical outcomes for survival. This analysis was two-sided. f, Scores of the p16h-sn CAF signature in relation to the indicated clinical predictive factors from the microarray data of patients with bladder cancer at Fukushima Medical University (metastasis, n = 114 (negative), n = 13 (positive); recurrence, n = 90 (negative), n = 31 (positive); pathological T stage, n = 64 (pTa), n = 45 (pT1), n = 21 (≥pT2); grade, n = 38 (low), n = 61 (high); muscle invasion, n = 97 (negative), n = 39 (positive); tumor size, n = 86 (≤3 cm), n = 48 (≥3 cm); number of tumors, n = 56 (single), n = 80 (multiple)). An unpaired t test was performed for d and f. One-way ANOVA with the Games–Howell test was performed for b and with Tukey’s test for d and f. Cox proportional hazards analysis was performed for e. Data in e are presented as hazard ratios with 95% confidence intervals. For all box plots, the horizontal line in the box indicates the median value. The lower and upper bounds correspond to the 25th and 75th percentiles, respectively. The cross mark indicates the mean value. Whiskers extend to the maximum and minimum values, with points outside this range drawn individually.
