Extended Data Fig. 2: Related Fig. 2; Single-cell RNA sequence analysis of p16h cells in aged male mouse bladder. | Nature Aging

Extended Data Fig. 2: Related Fig. 2; Single-cell RNA sequence analysis of p16h cells in aged male mouse bladder.

From: Preexisting senescent fibroblasts in the aged bladder create a tumor-permissive niche through CXCL12 secretion

Extended Data Fig. 2

a, Scheme of scRNA libraries preparation using non-immune Tomato− (p16l) and Tomato+ (p16h-sn) bladder cells from a 25-month-old male p16-CreERT2-tdTomato mouse. b, Gating strategy for sorting non-immune Tomato− (p16l) and Tomato+ (p16h-sn) bladder cells. c, Representative Immunofluorescence images of aged (22–26-month-old) male mouse bladder as the indicated staining. White arrowheads indicate double positive cells for both Tomato and each indicated protein. Scale bar, 100 μm. d, Percentage of cell type among Tomato+ (p16h-sn) cells. (n = 8 for each cell type). e, qPCR using the primer of Cxcl12 on sorted fibroblasts from young (7–12-week-old) male p16-CreERT2-Tomato mice. (n = 6). Paired t-test was performed for e. Data in d, e are presented as mean values ± SEM. All the t-test were two−side.

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