Extended Data Fig. 2: Expression of mito-Dendra2 in HSCs and analysis of their phenotype during bone marrow transplantation.
(a) Mitochondrial mass in young and aged HSPCs. Representative histogram of mito-Dendra2 for ST-HSC, MPP, LS−K, and Lin−. (b) Mitochondrial DNA copy number in mito-Dendra2 Low and High HSCs (P = 0.0118, n = 6). (c) EPCR expression in aged ESLAM-HSCs by mitochondrial mass. Relative MFI of EPCR (P = 0.0001, n = 6). (d) Complete blood count post-BMT (n = 6, 8 for Low and High groups). P-values by week: white blood cell - 4 (0.0015), 8 (0.4187), 12 ( < 0.0001), 16 (0.0529); red blood cell - 4 (0.8886), 8 (0.8816), 12 (0.0300), 16 (0.8269); hemoglobin - 4 (0.4995), 8 (0.3988), 12 (0.0037), 16 (0.8101); hematocrit - 4 (0.9067), 8 (0.4799), 12 (0.0092), 16 (0.4611); platelet - 4 (0.0672), 8 (0.8458), 12 (0.5581), 16 (0.6478). (e-g) Proportion of mito-Dendra2+ cell in LSK and LS−K fractions at 16 weeks post-BMT (Low: n = 6, High: n = 8). (e) HSPCs (Extended Data Fig. 1a). P-values: ST-HSC (0.1639), MPP (0.5754), LSK (0.1102), LS−K (0.5728), Lin− (0.5853). (f) HSPCs (Extended Data Fig. 1b). P-values: ST-HSC (CD34+CD135−) (0.2781), MPP (CD34+CD135+) (0.4255), CMP (0.6850), GMP (0.5686), MEP (0.5608). (g) MPP2 (CD150+CD48− ESLAM-HSCs). P = 0.1084. (h) mito-Dendra2 expression in donor-derived cells in the HSC at 16 weeks post-BMT. Due to the lower number of donor-positive cells in the HSC fraction originating from mito-Dendra2 Low cells, the sample size is smaller compared to that of mito-Dendra2 High cells (n = 3, 7 for mito-Dendra2 Low and High). P = 0.1510. (i) Proportion of donor-derived cells post-BMT in young mice (ref. 26; n = 5, 4 for Young-Low and Young-High). P-values: SLAM (0.0374), ST-HSC (0.1513), MPP (0.0975). Note: All charts show mean ± SD. Statistical analysis: two-tailed Student’s t-test (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns = not significant).
