Extended Data Fig. 5: Macrophages with a senescent phenotype disrupt muscle homeostasis in the Quadriceps of OA joints. | Nature Aging

Extended Data Fig. 5: Macrophages with a senescent phenotype disrupt muscle homeostasis in the Quadriceps of OA joints.

From: Senescent macrophages induce ferroptosis in skeletal muscle and accelerate osteoarthritis-related muscle atrophy

Extended Data Fig. 5

(a) The relative SASP mRNA expression levels (n = 5). (b) Representative Dystrophin staining images and mean CSA analysis of Quadriceps in mice with or without DT treatment at S12(n = 5). (c) Scheme of C12FDG+/− macrophages transplantation into the Quadriceps of mice in vivo. (d) The flow cytometry profiles and corresponding quantitative results of living C12FDG+/− macrophages at 2- and 4-weeks post-transplantation. (n = 3). (e) The SASP mRNA expression levels, (n = 5), (f) WGA staining and mean CSA analysis of the Quadriceps from C12FDG+/− macrophage recipient mice at D12. (n = 6). Data are presented as mean±s.e.m. unless otherwise noted, all statistical tests were two-sided unless otherwise noted, and n represented biological replicates unless otherwise noted. Scale bar=50 μm. Student’s t-test (a, b, n-p).

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