Extended Data Fig. 9: Systemic supplementation of CoQ10 can enhance Quadriceps quality in OA joints of mice and mitigate cartilage damage.
(a) The HMGCR expression and quantification results of western blotting analysis (n = 3), and (b) the level of ubiquinone (n = 5), and (c) the quantification of ubiquinol (n = 5) in Quadriceps of mice with or without L-Asp supplementation at S12 and D12. (d) The HMGCR expression and quantification results of western blotting analysis (n = 3), and (e) the level of ubiquinone (n = 5), and (f) the quantification of ubiquinol (n = 5) in Quadriceps of mice with or without MHY-1485 intervention at S12 and D12. (g) The HMGCR expression and quantification results of western blotting analysis (n = 3), and (h) the level of ubiquinone (n = 5), and (i) the quantification of ubiquinol (n = 5) in Quadriceps of mice with or without D&Q intervention at S12 and D12. (j) Scheme of D&Q intervention in DMM mice with or without dysfunction of Quadriceps under BTX-A injection. (k) Representative Safranine O-Fast Green Staining images along with OARSI scores of joint cartilage in DMM mice with or without D&Q intervention and dysfunction of Quadriceps. (n = 6). Data are presented as mean±s.e.m. unless otherwise noted, all statistical tests were two-sided unless otherwise noted, and n represented biological replicates unless otherwise noted. Data are presented as mean±s.e.m. unless otherwise noted, all statistical tests were two-sided unless otherwise noted, and n represented biological replicates unless otherwise noted. Mann-Whitney U test (k). One-way ANOVA (a, c-e, g-i). One-way ANOVA with Tamhane’s T2 test (f). Kruskal-Wallis test (b). median (25% IQR, 75% IQR) (b, k).
