Fig. 4: Macrophages in the quadriceps of OA joints exhibit a senescent phenotype.
a, The top enriched terms of GO analysis for the significant DEGs in quadriceps of mice between D12 versus S12. b, The top enriched pathways of KEGG analysis for the significant DEGs in quadriceps of mice between D12 versus S12. c, The representative gene sets of GSEA for the DEGs in quadriceps of mice between D12 versus S12. d,e, Representative images of SA-β-gal+ macrophages and counting result (d), and immunofluorescence staining images of p16INK4a+ macrophages sorted from quadriceps tissue of mice at S12 and D12 and the analysis of MFI (n = 5) (e). f, Representative immunofluorescence images and quantitative results of F4/80+p16INK4a+ cell population in quadriceps cross-sections of mice at S12 and D12 (n = 5). g,h, The flow cytometry profiles and corresponding quantitative results of Ki67+ (g, n = 5) or γH2AX+ (h, n = 6) cells in living CD45+CD11b+F4/80+ macrophages within quadriceps tissue of mice at S12 and D1. i, Representative images and quantification analysis of F4/80+γH2AX+ cell population in quadriceps cross-sections of mice at S12 and D12 (n = 5). j, The flow cytometry profiles and corresponding quantitative results of MitoTracker intensity in γH2AX+ and γH2AX− macrophages isolated from quadriceps tissue of mice at D12 (n = 5). k,l, Representative images and the analysis results of Dil+ area (k) and LAMP2 intensity (l) were compared between γH2AX+ and γH2AX−macrophages isolated from quadriceps of mice at D12 (n = 5). m, Relative mRNA expression levels of SASP in macrophages isolated from quadriceps tissue of mice at S12 and D12 after D&Q treatment. Macrophages from each tested sample were sorted and pooled from a minimum of 10 individual mice (n = 3). Data are presented as mean ± s.e.m. unless otherwise noted; all statistical tests were two-sided unless otherwise noted; and n represents biological replicates unless otherwise noted. Student’s t-test (d–l). One-way ANOVA with Bonferroni test (m (Tnf), m (Il-6), m (Ccl6), m (Ccl2)). One-way ANOVA with Tamhane’s T2 test (m (Il-1β), m (Cxcl4), m (Ccl7), m (Ccl4)).
