Extended Data Fig. 2: Comparison of endogenous (CD45.2) and transferred (CD45.1) subsets in senolytic-treated old mice.

a. Graph shows the percentages of EOMES⁺CCL5⁺ cells originating from CD45.1⁺ cells in control (n = 11) and senolytic drug-treated (n = 10) mice. b-e. The ratio between young-transferred (CD45.1) and old-endogenous (CD45.2) Treg (CD4⁺FOXP3⁺; b), naïve (CD3⁺CD4⁺CD62L⁺CD44⁻; c), effector (CD3⁺CD4⁺CD62L⁻CD44⁺; d), and CD4⁺ PD1⁺ (e) cells in old control (Control; n = 11) and senolytic drug-treated mice (Senolytic; n = 10). Each dot represents the ratio in a single mouse. The right panels show the percentage of old-endogenous (CD45.2) and the young-transferred (CD45.1) cells of each subset in the old-control (Left) and old-senolytic (Right) mice. Bars represent mean ± SEM from three independent experiments. Data were analyzed using two-tailed unpaired (Left panels) or paired (Right panels) Student’s t-tests.