Extended Data Fig. 5: Phenotype of CD8 + TILs in mice with tumors.
(a–d) TIL DP8 cells only acquire exhausted phenotype at the late stage of tumor. (a) AT3 tumor growth (mm2) in Y- and A-WT mice; red squares indicate the days when tumors were processed for TIL phenotyping; (b) representative contour plots (left) and quantification (right panel) showing expression of exhaustion markers in DP8 cells in dLNs and TILs at different timepoints of tumor growth. (c) Frequency of DP8 cells in different memory subsets and (d) with CD3 or CD8 cells in dLNs and TILs of Y- and A- C57BL/6J mice at different time points of AT3 tumor growth. (e) unlike TIL CD8 + T cells, A-AT3 tumor LN CD8 T cells upregulate expression of effector molecules compared to naïve mouse LN CD8+ T cells. (f-i) Growth of i.p. challenged ID8-RFP ovarian cancer is increased in PeC of aged C57BL/6J mice (f) together with CD8+ T cells (g) and DP8 cells (h, numbers; and i, frequency). Shown is mean number or frequency ± SEM (f–i), where each symbol is for a single mouse, n = 5-6 (e), n = 10-11 (f, g), and n = 2-6 (h-i) mice per group. Experiments were reproduced three times. ** P < 0.01; *** P < 0.001 in unpaired Mann-Whitney test and Kruskal-Wallis test.
