Extended Data Fig. 2: Aging dysregulates T cells in a B-cell dependent manner.
(a) Frequency (upper) and numbers (lower panels) of IFNg+TNFa+ CD4+ and CD8+ cells in spleen of Y- and A- WT and BKO mice. (b) Frequency of splenic CD8+ T cells expressing CD39, Blimp1, ST2, CD103, CD73, KLRG1, TNFR2, and (c) exhaustion markers. (d–f) Representative dot plots of LN CD8+ T cells expressing Ly108 and CD69 (d), CD101 and Tox (e); CD39 and CD73 (f). Summary quantifications (frequency) of dot plots data are shown in the lower panel (d) or on the right (e). (g) Representative dot plot and frequency of LN CD8+ T cells expressing CD39 and CD73 in young (Y) and aged (A) BALB/cByJ mice. (h, i) Representative hierarchical dot plots showing expression of CD62L and CD44 in CD39 and CD73 sub-populations of CD8+ T cells from LN of Y-WT, A-WT, and A-BKO mice (h) and of exhaustion markers in Ly108 and CD69 expressing DP or SP39 cells in LN of A-WT mice (i). Shown are mean numbers or frequency ± SEM, where each symbol is for a single mouse, n = 3-6 mice per group (a–e and g). Experiments were reproduced three times. ** P < 0.01; *** P < 0.001 in unpaired Mann-Whitney test and Kruskal-Wallis test.
