Extended Data Fig. 9: R10.4.1 nanopore raw signals mapping.
From: Real-time raw signal genomic analysis using fully integrated memristor hardware
a, Recall, b, precision and c, F1 scores as a function of CAM threshold. We mapped 10,000 SARS-CoV-2 R10.4.1 raw signals (simulated by Squigulator) to the SARS-CoV-2 reference genome. A mapping result is considered correct if the genomic location identified by our method overlaps with the location determined by minimap2. As shown in the figure, when the CAM threshold is set to one, the hardware performs exact searching and cannot tolerate errors in nanopore sequencing signals, resulting in low recall but high precision. When the CAM threshold is set to around six, it becomes more robust to sequencing noise, improving recall while maintaining high precision. However, if the CAM threshold is too large, excessive false negatives are introduced, degrading both recall and precision. The optimal balance is achieved at a CAM threshold of six, where the F1 score reaches its peak at 99.4%.
