Fig. 3: Internalization of FAB02 is mediated by TfR1. | Communications Medicine

Fig. 3: Internalization of FAB02 is mediated by TfR1.

From: FORCE platform overcomes barriers of oligonucleotide delivery to muscle and corrects myotonic dystrophy features in preclinical models

Fig. 3

Representative images of WT and TfR1−/− HeLa cells (a) treated for 4 h with FAB02 labeled with AlexaFluor 647 (pseudocolored to green). Cells were stained with wheat germ agglutinin labeled with AlexaFlour 488 (membrane, pseudocolored to red,) and DAPI (nuclei). White bars within images indicate size. b WT (blue bars) and TfR1−/− (black bars) HeLa cells were treated for 24 h with FAB02 labeled with a pH-sensitive dye (CypHer5E). Cells were harvested and analyzed by flow cytometry for median fluorescent intensity normalized to the degree of labeling (Dye/Ab). Data are medians + SD. Each circle represents a technical replicate from two independent experiments. c Representative images of human cardiomyocytes treated for 30 min with FAB02 labeled with AlexaFluor 647 (pseudocolored to green), fixed, stained with DAPI (nuclei) and RAB5 (30 min sample, red). The image with merged AlexaFluor 647, RAB5, DAPI signals is an enlargement of the white boxed area. White bars within images indicate size.

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