Fig. 4: FDC1 suppresses Dmpk mRNA in skeletal and cardiac muscle of TfR1hu/mu mice to a greater extent than ASO1.
TfR1hu/mu mice were injected via tail vein on days 0 and 7 with vehicle (gray bars), 10 mg/kg ASO1 (red bars), or FDC1 delivering 10 mg/kg ASO-equivalent of ASO1 (blue bars) and sacrificed 14 days later for analysis of gene expression. Total RNA extracted from the indicated muscles was reverse-transcribed and Dmpk RNA expression was measured by qPCR using Ppib as the reference gene. Expression in each muscle from mice treated with FDC1 or ASO1 was normalized to Dmpk expression in the corresponding muscle of mice treated with vehicle. Data are means + SD; data were analyzed by one-way ANOVA followed by uncorrected Fisher’s LSD test, statistically significant differences from vehicle or between ASO1 and FDC1 are indicated with exact P values. Each circle represents an individual mouse.
