Fig. 8: Increased SOX10-MITF cfRNA in RCC patients after transition to acquired resistance. | Communications Medicine

Fig. 8: Increased SOX10-MITF cfRNA in RCC patients after transition to acquired resistance.

From: Acquired resistance to immune checkpoint therapy is caused by glycoprotein non-metastatic melanoma protein B signal cascade

Fig. 8

a Schematic presentation of nested real-time PCR analysis. b Nested qPCR standard curves: target cDNA template (23 μM) was 10-fold serially diluted, PCR-amplified for 15 cycles, and an aliquot (5% of total products) was then subjected to nested qPCR. Dilution factors are plotted against Cq value. c Plasma cfRNA at varying time points of acquired resistance patients (Pat #9, 19, and 20, Fig. 2b) and from three healthy donors (HD #1, 2, and 3) were quantified by nested qPCR for expression of GPNMB, MITF, SOX10, and β-ACTIN relative to GAPDH, and expressed as fold change to Week 0 of each patient (average, n = 2). Expression levels in HD are indicated by relative expression to the value at week 0 of Pat #9. Gray-filled bars represent time points for stable disease (SD) just before progressive disease PD (shown in black-filled bars). p values are shown, using Student’s t-test comparing gray- and black-filled bars in patients or #1 and #3 β-ACTIN in HD.

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