Fig. 1: Left ventricular functional data.

a Left ventricular ejection fraction (LVEF) from ligation through heart failure development, therapy, and for the post-therapy period. Time 0 refers to the day of injection for the AAV9-GFP (n = 4) and AAV9-cBIN1 (n = 6) treated cohorts. b Absolute LVEF from time of therapy to post-therapy week 5. c Left ventricular global longitudinal strain (GLS) from time of therapy to post-therapy week 5. All data were recorded in awake animals. In (a), two-way ANOVA mixed model was used to determine the statistical significance over time between two groups at day 14 (p = 0.2218), day 21 (p = 0.0327), day 28 (p = 0.0007) and day 35 (p = 0.0001). In (b and c), Mann–Whitney test is used to compute the statistical comparison between two groups (AAV9-GFP-treated group vs. AAV9-cBIN1-treated group) at post-therapy week 5 (LVEF: p = 0.0095 and GLS: p = 0.0095). In (b and c), Two-tailed paired t test is used to statistically compare the mean of the paired data between two different time points (pre-therapy vs. post-therapy week 5) within same group (for AAV9-GFP-treated group, LVEF: p = 0.0014 and GLS: p = 0.0228, and for AAV9-cBIN1-treated group, LVEF: p = 0.0005 and GLS: p = 0.0137). Data presented in (b and c) are shown as ‘mean’ and error bars are shown as ‘standard deviation’.