Fig. 7: Biosafety investigation of large-area mid-infrared (MIR) femtosecond (fs) laser keratotomy.
From: In vivo keratotomy by mid-infrared femtosecond laser resonant with amide vibrational mode
a Experimental photo of in vivo retinal function evaluation by electroretinography (ERG). b Typical waveform of ERG under scotopic condition, where a-wave is the first negative wave which reflects outer retinal function and b-wave is the first maximum positive wave which reflects inner retinal function. c Representative measured waveforms of ERG before and 8 days after large-area keratotomy. The difference of latency and amplitude between control and study groups are shown in (d) and (e) respectively. No significant latency and amplitude differences are found in the three groups after large-area ablation, which indicates the MIR fs laser keratotomy does not cause obvious retinal function abnormalities and obstruction to ocular optical pathway. f Schematic diagram of the visual cliff tests. g The normalized ratio of safe side at different postoperative time points. It shows that there are almost no abnormalities in visual behavior caused by the large-area MIR fs laser keratotomy. All data are means ± SEM. n.s. not significant.
