Fig. 8: RNA-seq of treated retinas revealed the therapeutic rescue of phototransduction genes in 3-month-old Cnga1⁻/⁻ mice. | Communications Medicine

Fig. 8: RNA-seq of treated retinas revealed the therapeutic rescue of phototransduction genes in 3-month-old Cnga1⁻/⁻ mice.

From: Gene augmentation therapy restores vision and preserves photoreceptors in a mouse model of CNGA1-retinitis pigmentosa

Fig. 8

A Volcano plot of differentially expressed genes between wild type and untreated retinas (|log2(fold change) |)> 0.58 and p < 0.05. B Volcano plot of differentially expressed genes between untreated and treated retinas (|log2(fold change) |)> 0.58 and p < 0.05. C GSEA revealed significant downregulation of phototransduction and photoreceptor cell maintenance pathways in untreated versus wild-type retinas. D GSEA showed upregulation of phototransduction and photoreceptor cell maintenance pathways in treated versus untreated retinas. E Relative expression levels of rod phototransduction-related genes in wild type, untreated, and treated retinas, as measured by real-time qPCR (n = 4). mRNA levels were normalized to Gapdh expression and presented as Mean ± SEM. Statistical significance determined by one-way ANOVA with Tukey’s post hoc test.

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