Extended Data Fig. 10: Leveraging enCRISPRa to upregulate ACTN2.
From: A transcriptional enhancer regulates cardiac maturation
Transfection of hPSC-CMs with sgRNAs targeting the 5’ border of the conserved ACTN2 enhancer region, upregulated ACTN2 protein. Relative protein expression compared to sgRNA1 is presented. (3 independent experiments were analyzed). Data are presented as mean values +/− SEM. All replicates are biological. The Shapiro-Wilk test was performed to assess normal distribution, and paired student parametric t-test (two-tailed) was used for all comparisons.
