Extended Data Fig. 9: FTY720, rather than its metabolites, suppresses Mmp2 transcription.
From: The TRPM7 chanzyme in smooth muscle cells drives abdominal aortic aneurysm in mice
(a) MMP2 transcripts in Ang II-stimulated SMC pretreated for 1 h with vehicle, FTY720 (3 μM) and its metabolites FTY720 (S)-P (3 μM) or FTY720 (R)-P (3 μM). n = 4-6. (b-c) Immunoblots of MMP2 in cells treated as indicated. n = 6-9. (d) MMP2 transcripts in saline or Ang II-infused mice treated with FTY720 (6 mg/kg/day) or FTY720 (S)-P (6 mg/kg/day). n = 6. (e-f) Immunoblots of MMP2 in mice treated as indicated. n = 5-6. (g) MMP2 transcripts in saline or PPE-perfused mice treated with FTY720 (6 mg/kg/day) or FTY720 (S)-P (6 mg/kg/day). n = 6. (h-i) Immunoblots of MMP2 in saline or PPE mice treated as indicated. n = 5-7. The differences were analyzed using two-way ANOVA, followed by Tukey’s post-hoc test (Extended Data Fig. 9a,b,d,e,g,h).
