Extended Data Fig. 5: Canrenone improves donor heart function with ex-vivo perfusion and transplantation after prolonged preservation.
From: Mineralocorticoid receptor phase separation modulates cardiac preservation
a, Canrenone dose titration with murine donor hearts cold (4 °C) preserved for 16 h followed by ex-vivo perfusion with Kreb buffer (n = 6/group). Tested canrenone dosages of 12.5 μM, 50 μM, and 200 μM are indicated.Preservation time titration of canrenone (50 μM) versus vehicle control (n = 5/group) with ex-vivo perfusion and conductance catheter assessment of cardiac b, contractility and c, relaxation. Transplanted donor heart d, contractility and e, relaxation (n = 8 for HTK+Veh, n = 9 for HTK+Canr) as well as f, circulating cardiac troponin I levels were compared following 16 h of cardiac preservation with canrenone versus vehicle control (n = 8/group). Cold preservation with HTK only, HTK + finerenone (50 μM) versus HTK + spironolactone (50 μM) (n = 8/group) with ex-vivo perfusion and conductance catheter assessment of cardiac g, contractility and h, relaxation. Data are presented as means ± SD. *P < 0.05 and **P < 0.01 by two-sided Mann-Whitney test.
