Extended Data Fig. 9: Canrenone reverses cold (4 °C) preservation induced transcriptomic changes in smooth muscle cells (SMC) and leukocytes (LK) within human donor hearts during storage.
From: Mineralocorticoid receptor phase separation modulates cardiac preservation
a. Cold preservation induced gene expression was identified by comparing human donor heart with 10 h of preservation compared to baseline. Red dots indicate upregulated genes in SMC population during cold storage while blue dots show the effect of canrenone on genes represented by the red dots. Light blue dots indicate downregulated genes in SMC population during cold storage while purple dots show the effect of canrenone on genes represented by the light blue dots. Volcano plot demonstrates that Canrenone reverses the cold preservation-induced global up- and downregulated genes in SMC. b. Heatmap of gene expression in SMC of human donor hearts cold (4 °C) preserved for 10 h versus baseline (n = 3/group). Juxtaposed to the right is the HTK+Canr versus HTK+Veh effect on gene expression following 10 h of cold (4 °C) preservation of human donor hearts (n = 3/group). c. Cold preservation induced gene expression was identified by comparing human donor heart with 10 h of preservation compared to baseline. Red dots indicate upregulated genes in LK population during cold storage while blue dots show the effect of canrenone on genes represented by the red dots. Light blue dots indicate downregulated genes in LK population during cold storage while purple dots show the effect of canrenone on genes represented by the light blue dots. Volcano plot demonstrates that Canrenone reverses the cold preservation-induced global up- and downregulated genes in LK. d. Heatmap of gene expression in LK of human donor hearts cold (4 °C) preserved for 10 h versus baseline (n = 3/group). Juxtaposed to the right is the HTK+Canr versus HTK+Veh effect on gene expression following 10 h of cold (4 °C) preservation of human donor hearts (n = 3/group).Baseline is where samples were collected in the back table immediately following preservation solution administration and removal of the donor heart from the surgical field.
