Extended Data Fig. 10: Pseudotime analysis showing the modulatory effect of canrenone on cold donor heart preservation associated gene expression.
From: Mineralocorticoid receptor phase separation modulates cardiac preservation
Density distribution plot showing the distribution of cells having differential transcriptome expression profile along pseudotime for cold (4 °C) preserved human hearts without reperfusion at a, baseline versus 10 h of preservation (n = 3/group) or b, for human hearts cold (4 °C) preserved for 10 h without reperfusion using HTK alone versus HTK + canrenone (CANR, n = 3/group). c, “Rolling wave” heatmap plot along pseudotime trajectory showing the differentially expressed genes ordered according to hierarchical clustering following 10 h of human heart preservation without reperfusion using HTK + VEH versus HTK + CANR (n = 4/group). P-values for pseudotime dependency using the Kolmogorov-Smirnov test are shown.
