Extended Data Fig. 2: Donor heart preservation is associated with increased MR protein expression.
From: Mineralocorticoid receptor phase separation modulates cardiac preservation
Increased MR protein expression is seen with normalization to baseline preservation time in a, human donor hearts at baseline, 4 and 10 h of 4 °C static preservation with HTK without reperfusion (n = 3/group) and b, murine donor heart after baseline and 16 h of 4 °C HTK preservation followed by ex-vivo perfusion (n = 4/group). c, MR immunostaining (purple) with DAPI (blue) in MRfl/fl versus Myh6CreERT2;MRfl/fl hearts with tamoxifen injection. Representative fluorescence images of n = 4/group. d, QRT-PCR analysis of cytokine transcript expression in transplanted donor hearts preserved for 16 h normalized to control MRfl/fl hearts at baseline time (n = 3). e, Immunostaining of CD45 (red) and Ly6C (red) with DAPI (blue) in transplanted hearts. Representative fluorescence images of n = 4/group. f. Quantitation of positive staining cell counts expressed as percentage of total cells averaged over 4 high powered (200x) fields (n = 4). Data are presented as means ± SD. *P < 0.05 and **P < 0.01 by two-sided Mann-Whitney test, Tukey’s corrections were used for multiple comparisons using one-way ANOVA.
