Extended Data Fig. 3: CD82 facilitates vascular inflammation.
(a) Representative images of echocardiography from Ctrl. or Cd82ΔEC mice challenged with LPS (5 mg/kg) at 18 h or 48 h after the LPS administration. (b) Cd82 ablation does not alter the basal permeability of skin vessels. Evans blue dye (30 mg/kg in PBS) was injected intravenously through the retro-orbital sinus into 8~12-week-old Cd82−/− mice or their WT littermates. The leakage of Evans blue dye into ear skin was assessed at 0, 30, or 60 min after the injection. Extravasated Evans dye was quantified and corrected with ear dry weight (mean±SEM; n=6 mice per group for 60 min and n=11 mice per group for 30 min). (c) Representative images of Evans blue dye leakage into skin of the ears from WT and Cd82−/− mice as well as from Ctrl. and Cd82ΔEC mice in Miles assay. (d) Body weight was measured daily after SARS-Cov-2 infection in Ctrl. or Cd82ΔEC mice (mean±SD; n=9 mice for Ctrl. group and n=8 mice for Cd82ΔEC group). (e) SARS-Cov-2 titer in control and Cd82ΔEC mice. Pulmonary tissues were harvested from Ctrl. or Cd82ΔEC mice, and SARS-CoV-2 nucleocapsid (N) gene expression is presented as its relative level after normalization with actin expression using the dCT method for all mice in the experiment (left panel) and only for those mice which succumbed to infection at the time of death (right panel). Nine mice from Ctrl. group and eight mice from Cd82ΔEC group were used for the study.
