Extended Data Fig. 1: Acute treadmill exercise heart gene expression timecourse.
From: Cardiac adaptation to endurance exercise training requires suppression of GDF15 via PGC-1α
a, Ppargc1a mRNA expression in the indicated tissues in WT and KO mice. b, Experimental design for the acute treadmill exercise timecourse experiment. Created with BioRender.com. c–h, Relative mRNA expression of the indicated genes in the heart at the indicated timepoints following a single 45-minute bout of treadmill exercise. For (a), statistical significance was assessed by two-sided two-way ANOVA followed by Sidak’s multiple comparisons test (two-sided). Exact P values: 0.041 (heart), 1 (liver), 1 (kidney), 0.65 (gastrocnemius), 0.79 (iWAT). Data are presented as mean±s.e.m. For (a), n = 5 biologically independent mice per group. For (c–h), n = 4 biologically independent mice per group. Significance symbols: * P < 0.05; N.S., not significant. For all data in this figure, a single experiment was performed without a separate biological replication cohort. Mouse strain and age: for (a), mice studied were 10 week old male mice of the indicated strain (WT vs. KO) and n per group as above. For (c-h), all mice used were C57BL/6 mice from The Jackson Laboratory (age relative to initiation of the experiment) of the indicated n per group.
