Extended Data Fig. 1: Cardiac function and glycogen handling in diabetic hearts.
a, Ratio of phosphorylated glycogen synthase (pGS Ser641) to total glycogen synthase (GS) is unchanged in T2D mouse hearts (n = 8 animals/group, molecular weight indicated in kilodaltons, kDa, analyzed by unpaired 2-sided T-test). b, Ratio of phosphorylated glycogen phosphorylase (pGP Ser14) to total glycogen phosphorylase (GP) is unchanged in in T2D mouse hearts (Ctrl: n = 8, T2D: n = 10 animals/group, analyzed by unpaired 2-sided T-test). c, Exemplar M-mode and flow Doppler echocardiography traces. d, Echocardiography M-mode-derived ejection fraction and fractional shortening are unchanged with diabetes in type 2 diabetic (T2D, 14 week high fat sugar diet) vs control (Ctrl) mice (%EF n = 10 animals/group. %FS Ctrl: n = 10, T2D: n = 9 animals/group, analyzed by unpaired 2-sided T-test). e, Glycogen content normalized to protein in adult rat cardiomyocyte lysate from Ctrl and streptozotocin (STZ)-induced diabetic rats (8 weeks post-STZ, Ctrl: n = 10, STZ: n = 9 animals/group, analyzed by unpaired 2-sided T-test, p = 0.0037). f, Ca2+ time constant of decay (Tau) in isolated adult cardiomyocytes from control and STZ rats (8 weeks post-STZ, Ctrl: n = 10, STZ: n = 9 animals/group, analyzed by unpaired 2-sided T-test, p = 0.0022). g, Correlation of cardiomyocyte glycogen content and Ca2+ time constant of decay (Tau) in control (blue) and STZ (pink) rat cardiomyocytes (unloaded cells; r, Pearson correlation coefficient). h, Gabarapl1 mRNA is lower in atrial appendage samples from patients with T2D (n = 12 patients/group, analyzed by unpaired 2-sided T-test, p = 0.043). i, GABARAPL1 protein content in fractionated atrial appendage samples from patients with T2D (Homog: Ctrl n = 9, T2D n = 4; Memb, Cyt, Memb:Cyt ratio: n = 5 patients/group, analyzed by unpaired 2-sided T-test). j, STBD1 protein content is increased in atrial appendage samples from patients with T2D (Ctrl: n = 9, T2D: n = 4 patients/group, analzyed by unpaired 2-sided T-test, p = 0.0418). Data presented as truncated violin plots with median and upper & lower quartiles indicated, or bar graphs mean +/- sem. *p < 0.05. Related to Fig. 2.
