Fig. 3: Knockdown of CPT2 supports endogenous VOC production.

a Expression of CPT2 quantified by RT-qPCR at the start of volatile organic compound (VOC) collection (time = 0) using biologically independent triplicate in a 6-well plate to estimate Biodome CPT2 expression levels. P-values were determined using a one-tailed t-test. b The expression of CPT2 was independently mapped across the four days of VOC sampling following siRNA-mediated knockdown in SK-OV-3 cells at equal passage number using biologically independent duplicate or triplicate as indicated with individual points. c Four VOCs not found in controls showing decreased abundance following the inhibition of β-oxidation via CPT2 knockdown, across 4 days of measurement. P values were determined using a one-tailed t-test and adjusted using the Benjamini-Hochberg procedure⁵¹. * denotes an adjusted p values < 0.05. Each time point was measured in biologically independent triplicate. Error bars represent the standard error of the mean. d Boxplots showing significant or trending toward significant decreases in endogenous VOC abundance following integration by unique mass and log₁₀ transformation. P-values, same as c. The median is indicated by the solid black line, and first and third quartiles are captured by the bounds of the box. Boxplot whiskers are defined as the first and third quartiles –/+ interquartile range times 1.5, respectively. Minimum and maximum values are captured by the lowermost and uppermost points, respectively, or whisker bound if no outliers are shown. The gray color represents the non-targeting siRNA pool while red indicates the CPT2 targeted siRNA pool.