Fig. 4: 3D automatic segmentation of vasculature using Open-3DULM and AMIRA (Amira 2019 software, FEI).
From: 3D transcranial ultrasound localization microscopy in awake mice: protocol and open-source pipeline

The watershed-like 3D vascular structure of Open-3DULM enables automatic segmentation of vessels, identifying the connections between branches and the main vessel. a Region 3 (Ach: anterior choroidal artery); Dlth (dorsolateral hypothalamus) and (b) Region 4 (MCA: middle cerebral artery). The identification of the MCA and ACH was performed by two radiologists.