Fig. 3: Specificity and spectrum of BZT’s intracellular antimicrobial activity. | npj Antimicrobials and Resistance

Fig. 3: Specificity and spectrum of BZT’s intracellular antimicrobial activity.

From: The Parkinson’s drug benztropine possesses histamine receptor 1-dependent host-directed antimicrobial activity against Mycobacterium tuberculosis

Fig. 3

a BZT showed a dose-dependent intracellular activity against Mtb in RAW 264.7 cells. b hMDMs infected with GFP-expressing Mtb were monitored for two weeks using live-cell imaging and automated GFP fluorescence area analysis. Mtb-infected hMDMs were treated with two-fold serial dilutions of BZT. Data were normalised to cells treated with the vehicle control (1% DMSO), defined as 0% inhibition, and the positive control (1 µg/mL RIF + INH, R + I), defined as 100% inhibition. c BZT demonstrated a dose-dependent inhibition of Mtb growth in hMDMs. d Comparison of Mtb growth in hMDMs isolated from three healthy donors. The statistical analysis was conducted by pooling data across all donors. BZT showed dose-dependent activity against STm in e THP-1 macrophages and f RAW 264.7 cells. Data represent mean ± SEM of at least two independent experiments. NT non-treated control.

Back to article page