Table 1 The details and primer/probe sequences of droplet digital PCR assays.
From: Monitoring circulating tumor DNA by recurrent hotspot mutations in bladder cancer
Mutation | Position | Fw primer sequence | Re primer sequence | Probe sequence for mutant | Probe sequence for wild type | Restriction enzyme | Annealing temperature | PCR cycle | Product ID |
|---|---|---|---|---|---|---|---|---|---|
TERT promoter 228 C > T | chr5: 1295,228 | – | – | – | – | CivQI | 62 | 50 | dHsaEXD72405942 |
TERT promoter 250 C > T | chr5: 1295,250 | – | – | – | – | CivQI | 62 | 50 | dHsaEXD46675715 |
PLEKHS1 promoter 590 G > A | chr10: 115,511,590 | gacctcttggcttcca | ctgcaaaattttccatttcca | tgcaattgtttaattgcaaaaaagc | tgcaattgttcaattgcaaaaaagc | HaeIII | 55 | 40 | - |
PLEKHS1 promoter 593 C > T | chr10: 115,511,593 | gacctcttggcttcca | ctgcaaaattttccatttcca | tgcaattattcaattgcaaaaaagc | tgcaattgttcaattgcaaaaaagc | HaeIII | 55 | 40 | - |
ADGRG6 promoter 206 G > A | chr6: 142,706,206 | tgcatatttcacatggac | atcctggagggagaatac | aggctctttgtatgtttatacaaag | aggctctttgtatgttcatacaaag | HaeIII | 55 | 50 | - |
ADGRG6 promoter 209 C > T | chr6: 142,706,209 | tgcatatttcacatggac | atcctggagggagaatac | aggctctttgtatattcatacaaag | aggctctttgtatgttcatacaaag | HaeIII | 55 | 50 | - |
WDR74 promoter 254 G > A | chr11: 62,609,254 | tcggatagaggacgtatcag | gcgaccgaagtaaaggtatc | ctacacttaatcttagccaaaaggc | ctacacttgatcttagccaaaaggc | HaeIII | 55 | 40 | - |
