Fig. 1: The model multiphase condensate system.
From: Biomolecular condensates with complex architectures via controlled nucleation
a, Condensates can have various architectures. This Article shows how condensates with complex architectures can be designed. b, The model condensates are prepared by mixing Cm-amylose, Q-amylose and DNA in a solution with a terpolymer stabilizer, salt and buffer (Methods). c, Electrostatically driven phase separation35,50 yields a dilute phase (phase 1) and condensates containing phase 2 surrounding phase 3 at 40 °C (Extended Data Fig. 1). d, Upon heating the condensates to 65 °C, this architecture is maintained. e, However, cooling the condensates back to 40 °C causes the nucleation of droplets of phase 2 in phase 3 (orange arrowheads) and droplets of phase 3 in phase 2 (yellow arrowheads). Not the temperature itself, but how this temperature is reached determines which (transient) architecture is obtained (Extended Data Fig. 2).
