Fig. 6: Control over cargo uptake rate via control of the condensate architecture.
From: Biomolecular condensates with complex architectures via controlled nucleation
a, Confocal images of the uptake of 100 nM GFP, −30GFP or DNA–GFP into condensates. b, Although GFP and −30GFP uptake by phase 3 is weak, DNA–GFP has a strong preference for moving into phase 3, independent of the size of the droplets (n > 50 per box plot; data are presented as mean, 25th and 75th percentiles and s.d.; Extended Data Fig. 9). c,d, Lowering the [KCl] concentration in the solution before (c) or after (d) droplet formation creates a simple architecture with a small phase 2–phase 3 interface or a complex architecture with larger interface, respectively. DNA–GFP uptake is substantially faster in small droplets of phase 3, which have a relatively large interface area. e, The uptake of DNA–GFP in the condensate with a complex architecture is over five times faster than that for the simple architecture. For both the simple and complex architectures, 12 condensates of similar size were used.
