Fig. 9: Body weight changes in neonatal and adult mice following JUAV infection.

A Body weight changes in neonatal mice following JUAV infection. Neonatal mice (control, n = 10; infected, n = 10) received a single intranasal instillation of 20 µL of brain homogenate from infected or uninfected (control) mice. Significant weight loss was observed in the infected neonatal mice starting at 7 days post-instillation. B Body weight changes in adult mice following JUAV infection. Adult mice (control, n = 5; infected, n = 15) received daily intranasal administrations over three consecutive days. Significant weight loss was observed only in the infected group, coinciding with severe clinical signs at 35 days post-infection. Statistical analysis for neonatal mice (A) was performed using two-way ANOVA with Bonferroni correction for multiple comparisons (***p < 0.001; p < 0.01; p < 0.05). Statistical analysis for adult mice (B) was performed using two-way ANOVA with Tukey’s post hoc test (***p < 0.001; **p < 0.01; *p < 0.05). Weight measurements were recorded from three independent cohorts of mice.