Fig. 3: Workflow for ultrasound localisation microscopy.
From: Advanced ultrasound methods to improve chronic kidney disease diagnosis
A B-mode images of a contrast bolus are acquired along with either contrast-enhanced imaging or as shown here with a slow-flow Doppler mode (SMI, Canon) to better detect microbubbles. B The image of tissue background is used for motion estimation and compensation. Here motion estimation was performed with the function imregdemons provided by Matlab (Mathworks, Natick, MA, USA). C Microbubbles are detected and localised in the contrast image. D Tracking of microbubbles and visualizing the tracks results in the images of super-resolved vessel. Here, an image of microbubble counts is shown. Images are from a study at RWTH Aachen University with ethical approval by the local review board.
