Fig. 4: Comparing the spiking activity of chronically tetrodotoxin (TTX)-treated vs.
From: Robust self-supervised denoising of voltage imaging data using CellMincer
control hPSC-derived neurons with raw and CellMincer-denoised Optopatch voltage imaging data. a Raw and denoised versions of a sample frame, colored with the neuron components identified in their corresponding datasets. b Corresponding ROI-averaged single-neuron traces detected in both versions of the above frame. c Spike count distributions, separated by neuron population and stimulation intensity. Spikes were identified in each detected neuron’s trace and binned by their stimulation intensity. The separation between the sets of green (TTX-treated) and purple (Control) boxplots in each respective plot indicates the degree to which we were able to identify the difference in spiking activity in the data. d Detected neuron counts in the raw and denoised versions of each dataset. e Statistical power of the Wilcoxon Rank Sum test applied to the neuron population differentiation hypothesis, reported as the negative logarithm of its p-value.
