Fig. 2: Two independent 3D reconstruction data sets including representative Profftella cells.

A, E Reconstructions from 200 consecutive 100 nm-thick images obtained through SBF-SEM, showing all Profftella cells within each cubic region. Each side of the cubic blocks is 20 μm in length. Different Profftella cells are colored differently. B, F These images show representative Profftella cells where the entire cell is contained within the respective resin blocks in A and E. The planes (XY, XZ, YZ axes) of the cubic region are also displayed. C, G Enlarged views of each Profftella cell shown in B and F. The cell surfaces are displayed transparently, and numerous internal tube structures are color-coded for distinction. The centerline tracings of the cells, shown in the upper right corner of each Fig., indicate that the cells are single-stranded. The length of each Profftella cell was approximately 49 μm (C) and 81 μm (G). D, H Multiple tube structures present inside each cell shown in C and G. All tubes in D and H are individually displayed in Supplementary Figs. 2, 3.