Table 1 The growth and phenology behavior of (RNAi) transgenic Populus transgenotypes.

From: Nature’s Master of Ceremony: The Populus Circadian Clock as Orchestrator of Tree Growth and Phenology

Transgenotype

Significant growth changes

compared to WTT89

Significant phenological changes

compared to WTT89

 

Ht.

Diam.

CCI

Bs.

Bb. (ap)

Bb. (lat)

Photoreceptor-related

pCCR2::LUC PHYA1-1

- (FE1)

- (FE1)

    

pCCR2::LUC PHYA22-2

- (PPE), - (FE1)

- (PPE), + (FE1)

+ (FE1)

D (FE1)

  

PHYB-12

+ (PPE), - (FE2)

+ (FE2)

    

PHYB2KO-6*

+ (PPE)

     

ztl-5

- (GCE1)

- (GCE1)

- (GCE1)

   

ztl-7

+ (FE1)

 

+ (GCE1)

   

fkf1-11 ©

- (FE1)

- (FE1)

- (FE1)

A (FE1)

  

lhy PHYA1-32 ©

pCCR2::LUC WT (T89)

prr7-5 ©

fkf1-10 ©

ztl-3

Core clock-related

lhy-10

- (GCE1), - (PPE), + (FE1)

- (GCE1), + (FE1)

 

D (FE1)

  

toc1-5

+ (FE1)

+ (FE1)

    

TOC1_OX-7* ©

+ (FE1)

     

TOC1_OX-4* ©

GI-related

gi-6 ©

- (PPE)

     

gi-13 ©

+ (FE1)

- (PPE)

+ (FE1)

D (FE1)

  

GILOX12*

- (FE2)

     

GIOX-12*

- (FE2)

     

AtGA20ox-related

pMEE1::AtGA20ox1-10 (T89)* ©

- (GCE4), + (FE2)

  

D (GCE4)

A (GCE4)

A (GCE4)

pMEE2::AtGA20ox1-2 (T89)* ©

+ (PPE), - (GCE4), + (FE2)

+ (PPE), - (GCE4), + (FE2)

 

D (GCE4)

 

A (GCE4)

pMEE2::AtGA20ox1-11 (T89)* ©

- (GCE4), + (FE2)

+ (FE2)

 

D (GCE4)

A (GCE4)

A (GCE4)

pMEE2::AtGA20ox1-5 (T89)* ©

- (GCE4)

  

D (GCE4)

 

A (GCE4)

pMEE1::AtGA20ox1-5 (Elite)* ©

+ (FE2)

- (GCE4), - (FE2)

 

A (GCE4)

D (GCE4)

D (GCE4)

pMEE1::AtGA20ox1-8 (Elite)* ©

+ (GCE4)

+ (GCE4)

 

A (GCE4)

D (GCE4)

D (GCE4)

pMEE2::AtGA20ox1-5 (Elite)* ©

+ (GCE4)

+ (GCE4)

 

A (GCE4)

D (GCE4)

D (GCE4)

pMEE2::AtGA20ox1-6 (Elite)* ©

+ (GCE4)

+ (GCE4)

 

A (GCE4)

D (GCE4)

D (GCE4)

pMEE1::AtGA20ox1-3 (T89)* ©

   

D (GCE4)

A (GCE4)

A (GCE4)

pMEE1::AtGA20ox1-8 (T89)* ©

   

D (GCE4)

A (GCE4)

A (GCE4)

pMEE1::AtGA20ox1-4 (Elite)* ©

   

A (GCE4)

D (GCE4)

D (GCE4)

pMEE2::AtGA20ox1-10 (Elite)* ©

   

A (GCE4)

D (GCE4)

D (GCE4)

CO/FT-related

CO1OX-13*

+ (FE2)

     

CO2OX-11*

- (FE2)

     

ft-16

- (FE1), - (GCE2), + (GCE3), - (FE2)

- (FE1), + (FE2)

- (FE1)

A (FE1), A (GCE3)

  

ft-7

- (FE2)

+ (FE2)

    

EBI1-related

ebi1-2 ©

+ (GCE1), + (PPE), + (FE1), + (FE2)

+ (GCE1), + (PPE), + (FE1)

    

ebi1-3 ©

+ (GCE1), + (FE1), + (FE2)

+ (GCE1), + (FE1)

+ (FE1)

   

ebi1-14 ©

+ (GCE1), + (FE1), + (FE2)

+ (FE1), - (FE2)

    

ebi2-7 ©

- (PPE), - (FE1)

- (PPE)

- (FE1)

   

ebi2-8 ©

- (PPE)

- (PPE)

- (FE1)

   

ebi2-6 ©

+ (FE1)

+ (FE1)

    

Bud & flowering-related

lfy-4

+ (FE2)

     

svp-3

+ (FE2)

     

SVPOX-6*

+ (FE2)

     

SVPOX-7*

- (FE2)

     

rcar-10

+ (FE2)

     

RCAROX-18*

+ (FE2)

     

msi-1a

 

tem1B-5*

 

OPR3-related

opr3-15 ©

+ (PPE), + (GCE2)

     

opr3_ft-3 ©

- (FE2)

+ (FE2)

    

opr3_ft-12 ©

- (GCE2)

     

opr3-7 ©

opr3-11 ©

opr3_ft-7 ©

AOS-related

aos-1 ©

   

D (GCE3)

  

aos-10 ©

+ (FE2)

  

D (GCE3)

  

aos-13 ©

+ (FE2)

+ (PPE)

 

D (GCE3)

D (GCE3)

 

aos_ft-10 ©

+ (GCE3)

- (GCE3)

 

A (GCE3)

 

D (GCE3)

aos_ft-1 ©

- (FE2)

+ (FE2)

 

A (GCE3)

  

aos_ft-13 ©

- (GCE3), - (FE2)

  

A (GCE3)

 

D (GCE3)

WT-related

      

WT (Elite)*

+ (PPE), + (GCE4)

+ (PPE)

 

A (GCE4)

D (GCE4)

D (GCE4)

WT (Elite-884056)*

      
  1. The name stands for either an experiment done in growth chambers (GCE), the phenotyping platform (PPE), or the field (FE). Ht., Diam., CCI, Bs. and Bb. stand for the height, diameter, chlorophyll content index, bud set, and bud burst, respectively. Measures of significance are symbolized as + and – for positive and negative significant differences between the growth or phenology of a transgenotype and WTT89 (i.e., clock mutants with + or – grow significantly faster or slower, respectively. Likewise, clock mutants with D or A display a significant delay or advance, than WTT89, respectively). Non-significant results are abbreviated as ns. Transgenotypes introduced here for the first time in the literature are marked with a © symbol. Overexpressing and knockdown transgenotypes are marked by an asterisk or underlined, respectively.