Fig. 5: Machine learning-driven analysis of the kinetics of heavy water uptake by the resistant E. coli strain.

Discriminant function analysis (DFA) of the kinetic incorporation of D2O-derived D isotope by metabolically active TMP-resistant cells cultured in the growth medium containing 4 µg/mL TMP (2 × MIC) in 50% D2O, highlighting progressive incorporation of heavy deuterium atoms into cellular biomass from 0, 1, 2, and 4 h.